pichia pastoris expression vector ppiczac (Thermo Fisher)
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pichia pastoris expression vector ppiczac
Pichia Pastoris Expression Vector Ppiczac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppiczac+vector/pm36660811-235-54-59
Average 90 stars, based on 1 article reviews
Pichia Pastoris Expression Vector Ppiczac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppiczac+vector/pm36660811-235-54-59
Average 90 stars, based on 1 article reviews
pichia pastoris expression vector ppiczac - by Bioz Stars,
2026-11
90/100 stars
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Plasmid Preparation:Article Title: Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris. Article Snippet: The endo-1,4-b-xylanase gene xyn11a from Fusarium oxysporum, member of the fungal glycosyl hydrolase (GH) family 11, was cloned and expressed in Pichia pastoris.. The mature xylanase gene, which generates after the excision of one intron and the secreting signal peptide, was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPICZaC.. The final construction was integrated into the genome of the methylotrophic yeast P. pastoris X33 and the ability to produce xylanase activity was evaluated in flask cultures. Article Title: Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia. Article Snippet: Xyloglucan plays an important structural role in primary cell walls, possibly tethering adjacent microfibrils and restraining cell expansion.. There is therefore considerable interest in understanding the role of xyloglucan endotransglucosylase/hydrolases (XTHs), which are encoded in Arabidopsis by a 33-member gene family.. We compared the key catalytic properties of two very different Arabidopsis XTHs (heterologously produced in Pichia), both of which are aluminium-repressed. Article Title: A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface. Article Snippet: Madin Darby canine kidney (MDCK) epithelial cells were purchased from RIKEN Bioresource Center (Ibaraki, Japan) and were maintained in MEM supplemented with 10% foetal bovine serum. .. A plasmid for expression of His6t-S-CD pseudozymogen (pPICZ-His6t-S-CD) in the methylotrophic yeast Pichia pastoris has been constructed using the Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Article Title: Recombinant expression of biologically active murine soluble EPCR. Article Snippet: Endothelial cell protein C receptor (EPCR) downregulates the coagulation system and prevents thrombosis by binding to protein C/activated protein C (APC) and factor VII/activated factor VII (VIIa).. Recombinant APC and factor VIIa have been shown to be useful in a variety of clinical conditions.. Murine models could prove extremely helpful in order to study in vivo actions of these drugs. Article Title: Expression, purification and characterization of a human single-chain Fv antibody fragment fused with the Fc of an IgG1 targeting a rabies antigen in Pichia pastoris. Article Snippet: a Department of Biotechnology, Institute of Life Science and Biopharmacology, Guangdong Pharmaceutical University, 280 Outer Ring Road East, Guangzhou Higher Education Mega Center, Guangzhou 510006, China b Department of Biological Engineering, College of Pharmacy, Jilin University, 1266 Fu Jin Road, Changchun 130021, China c Guangdong Provincial Key Laboratory of Bio-Engineering Medicine, National Engineering Research Centre of Genetic Medicine, Guangzhou, Guangdong 510632, China Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Expressing:Article Title: Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris. Article Snippet: The endo-1,4-b-xylanase gene xyn11a from Fusarium oxysporum, member of the fungal glycosyl hydrolase (GH) family 11, was cloned and expressed in Pichia pastoris.. The mature xylanase gene, which generates after the excision of one intron and the secreting signal peptide, was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPICZaC.. The final construction was integrated into the genome of the methylotrophic yeast P. pastoris X33 and the ability to produce xylanase activity was evaluated in flask cultures. Article Title: A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface. Article Snippet: Madin Darby canine kidney (MDCK) epithelial cells were purchased from RIKEN Bioresource Center (Ibaraki, Japan) and were maintained in MEM supplemented with 10% foetal bovine serum. .. A plasmid for expression of His6t-S-CD pseudozymogen (pPICZ-His6t-S-CD) in the methylotrophic yeast Pichia pastoris has been constructed using the Sequencing:Article Title: Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia. Article Snippet: Xyloglucan plays an important structural role in primary cell walls, possibly tethering adjacent microfibrils and restraining cell expansion.. There is therefore considerable interest in understanding the role of xyloglucan endotransglucosylase/hydrolases (XTHs), which are encoded in Arabidopsis by a 33-member gene family.. We compared the key catalytic properties of two very different Arabidopsis XTHs (heterologously produced in Pichia), both of which are aluminium-repressed. Construct:Article Title: A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface. Article Snippet: Madin Darby canine kidney (MDCK) epithelial cells were purchased from RIKEN Bioresource Center (Ibaraki, Japan) and were maintained in MEM supplemented with 10% foetal bovine serum. .. A plasmid for expression of His6t-S-CD pseudozymogen (pPICZ-His6t-S-CD) in the methylotrophic yeast Pichia pastoris has been constructed using the Amplification:Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Clone Assay:Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Article Title: Recombinant expression of biologically active murine soluble EPCR. Article Snippet: Endothelial cell protein C receptor (EPCR) downregulates the coagulation system and prevents thrombosis by binding to protein C/activated protein C (APC) and factor VII/activated factor VII (VIIa).. Recombinant APC and factor VIIa have been shown to be useful in a variety of clinical conditions.. Murine models could prove extremely helpful in order to study in vivo actions of these drugs. Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Recombinant:Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Transformation Assay:Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. |